| 产品概述: |
Complement component 4 is a protein involved in the complement system.It is cleaved into proteins 4a and 4b. C4d is the degradation product of the activated complement factor C4, a component of the classical complement cascade which is typically initiated by binding of antibodies to specific target molecules.
Following activation and degradation of the C4 molecule, thio-ester groups are exposed which allow transient, covalent binding of the degradation product C4d to endothelial cell surfaces and extracellular matrix components of vascular basement membranes near the sites of C4 activation. C4d is also found in intracytoplasmic vacuoles of endothelial cells. Covalent binding renders C4d a stable molecule that can easily be detected by immunohistochemistry. Detection of C4d is regarded as an indirect sign of an antibody response.
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| 特异性: |
The antibody is a Goat polyclonal antibody raised against C4. It has been selected for its ability to recognize C4 in ELISA Kit and western blotting.
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| 使用方法: |
WB=1:100-500 ELISA=1:500-1000
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
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| 稳定性: |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
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| 存储: |
Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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| 存储缓冲液: |
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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| 注意事项: |
This product is for research use only, not for diagnostic or therapeutic use.
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