| 产品概述: |
Transferrin is a blood plasma protein for iron ion delivery that, in humans, is encoded by the TF gene. Transferrin is a glycoprotein that binds iron very tightly but reversibly. Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h). Transferrin has a molecular weight of around 80 kiloDaltons and contains 2 specific high-affinity Fe(III) binding sites.
The affinity of transferrin for Fe(III) is extremely high (1023 M-1 at pH 7.4)[citation needed] but decreases progressively with decreasing pH below neutrality.In humans, transferrin consists of a poly peptide chain containing 679 amino acids. It is a complex composed of alpha helices and beta sheets to form two domains.
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| 特异性: |
The antibody is a Goat polyclonal antibody raised against TF. It has been selected for its ability to recognize TF in ELISA Kit and western blotting.
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| 使用方法: |
WB=1:100-500 ELISA=1:500-1000
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
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| 稳定性: |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
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| 存储: |
Shipped at 2-8°C. Upon delivery aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
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| 存储缓冲液: |
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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| 注意事项: |
This product is for research use only, not for diagnostic or therapeutic use.
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