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Human Peste des Petits Ruminants Virus antibody (PPRV-Ab) ELISA Kit


种属

Human (Homo sapiens)

UniProt号

N/A

缩写

PPRV-Ab

别名

N/A

线性范围

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灵敏度

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样本类型

Serum, Plasma, Other biological fluids

检测方法

Indirect ELISA

分析方法

Qualitative

反应时间

1-3h

样本体积

1-200 μL

检测波长

450 nm

货号

AE64493HU

规格

96T 48T 96T*5 96T*50

价格

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货期

3-5 working days

说明书

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原理:
The microtiter plate provided in this kit has been pre-coated with PPRV-Ag. Samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated Anti-Human PPRV-Ab and incubated. Then substrate solutions are added to each well. Only those wells that contain PPRV-Ab and enzyme-conjugated Anti-Goat PPRV-Ab will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. 
产品概述:
Ovine rinderpest, also commonly known as peste des petits ruminants (PPR), is a contagious disease primarily affecting mainly goats and sheep; however, camels and wild small ruminants can also be affected. PPR is currently present in North, Central, West and Eastern Africa, the Middle East, South Asia. It is caused by Small ruminants morbillivirus in the genus Morbillivirus, and is closely related to, among others, rinderpest morbillivirus, measles morbillivirus and canine morbillivirus (previously known as canine distemper virus). The disease is highly contagious, and can have a 80-100% mortality rate in acute cases in an epidemic setting. This virus does not infect humans.

组份:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 12 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

特异性:
This assay has high sensitivity and excellent specificity for detection of Human PPRV-Ab. No significant cross-reactivity or interference between Human PPRV-Ab and analogues was observed.
回收率:
Matrices listed below were spiked with certain level of recombinant Human PPRV-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of Human PPRV-Ab in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

精密度:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
线性:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human PPRV-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

稳定性:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
样本收集及储存:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
注意事项:
Store at 2-8°C. Please refer to Instruction Manual.