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鸡传染性法氏囊病病毒VP2抗体 (IBDV-VP2-Ab) ELISA Kit
Chicken Infectious bursal disease virus VP2 antibody (IBDV-VP2-Ab) ELISA Kit


种属

Chicken (Gallus)

UniProt号

N/A

缩写

IBDV-VP2-Ab

别名

N/A

线性范围

Request Information

灵敏度

Request Information

样本类型

Serum, Plasma, Other biological fluids

检测方法

Competitive ELISA

分析方法

Qualitative

反应时间

1-3h

样本体积

1-200 μL

检测波长

450 nm

货号

AE64466CH

规格

96T 48T

价格

查询

货期

3-5个工作日

说明书

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原理:
This assay employs the indirect competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IBDV-VP2. Standards or samples are then added to the appropriate microtiter plate wells with a antibody specific for IBDV-VP2 and incubated. After washing, HRP-Conjugated is added to the wells. Following a wash to remove any unbound HRP-Conjugated reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IBDV-VP2-Ab bound in the initial step. The color development is stopped and the intensity of the color is measured.

产品概述:
Infectious bursal disease, IBD (also known as Gumboro disease, infectious bursitis and infectious avian nephrosis) is a highly contagious disease of young chickens caused by infectious bursal disease virus (IBDV), characterized by immunosuppression and mortality generally at 3 to 6 weeks of age. The disease was first discovered in Gumboro, Delaware in 1962. It is economically important to the poultry industry worldwide due to increased susceptibility to other diseases and negative interference with effective vaccination. In recent years, very virulent strains of IBDV (vvIBDV), causing severe mortality in chicken, have emerged in Europe, Latin America, South-East Asia, Africa and the Middle East. Infection is via the oro-fecal route, with affected bird excreting high levels of the virus for approximately 2 weeks after infection.

组份:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 12 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

特异性:
This assay has high sensitivity and excellent specificity for detection of Chicken IBDV-VP2-Ab. No significant cross-reactivity or interference between Chicken IBDV-VP2-Ab and analogues was observed.
回收率:
Matrices listed below were spiked with certain level of recombinant Chicken IBDV-VP2-Ab and the recovery rates were calculated by comparing the measured value to the expected amount of Chicken IBDV-VP2-Ab in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

精密度:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
线性:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Chicken IBDV-VP2-Ab and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

稳定性:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
样本收集及储存:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
注意事项:
Store at 2-8°C. Please refer to Instruction Manual.