首页 // 产品中心 // 蛋白
 

Recombinant Human Histone-lysine N-methyltransferase EZH2 (EZH2) Protein
Recombinant Human Histone-lysine N-methyltransferase EZH2 (EZH2) Protein


生产种属

Human (Homo sapiens)

来源

Prokaryotic expression

宿主

E. coli

同义词

EZH2; ENX-1; ENX1; EZH1; EZH2b; KMT6; KMT6A; WVS; WVS2

浓度

1 mg/mL

形态

Lyophilized or Liquid

纯度

> 90% as determined by SDS-PAGE

序列

Met446-Met751 (Q15910)

标签

6*His-tag

纯化方式

Affinity purification

分子量

42 kDa

应用

Western blotting,ELISA;SDS-PAGE;Antigen;

货号

AP50294HU

规格

100μg 500μg

价格

查询

货期

3-5 weeks

说明书

登陆
产品概述:
Enhancer of zeste homolog 2 (EZH2) is a histone-lysine N-methyltransferase enzyme (EC 2.1.1.43) encoded by EZH2 gene, that participates in DNA methylation and, ultimately, transcriptional repression. EZH2 catalyzes the addition of methyl groups to histone H3 at lysine 27, by using the cofactor S-adenosyl-L-methionine. Methylation activity of EZH2 facilitates heterochromatin formation thereby silences gene function. Remodeling of chromosomal heterochromatin by EZH2 is also required during cell mitosis. EZH2 is the catalytic subunit of the Polycomb repressive complex 2 (PRC2). EZH2's catalytic activity relies on its formation of a complex with at least two other PRC2 components, SUZ12 and EED. As a histone methyltransferase (HMTase), EZH2's primary function is to methylate Lys-27 on histone 3 (H3K27me) by transferring a methyl group from the cofactor S-adenosyl-L-methionine (SAM), although recent studies have indicated that it is also capable of methylating non-histone proteins. EZH2 is capable of mono-, di-, and tri-methylation of H3K27 and has been associated with a variety of biological functions, including transcriptional repression and activation, hematopoiesis, development, and cell differentiation.

使用方法:
Reconstitute in PBS or others.
稳定性:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

存储:
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.

存储缓冲液:
PBS (58mM Na2HPO4, 17mM NaH2PO4, 68mM NaCl, pH7.). 5 % trehalose and 5 % mannitol are added as protectants before lyophilization.
注意事项:
This product is for research use only, not for diagnostic or therapeutic use.