| 产品概述: |
The his-tag is a series of six to nine histidine residues generally fused to either the carboxy or amino terminus of a recombinant protein. The small size of the his-tag, compared with other common epitope tags, makes it less likely to obstruct the target protein’s structure or function and more suitable to use under denaturing conditions. The string of histidine residues binds to several types of immobilized metal ions, including nickel, cobalt and copper. The binding to metal ions under specific buffer conditions, allows for the simple purification and detection of his-tagged proteins.
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| 特异性: |
His-tag Polyclonal Antibody detects His-tagged recombinant proteins or His-tagged proteins overexpressed in cells.
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| 使用方法: |
WB=1:1000-3000 ELISA=1:5000-20000 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
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| 稳定性: |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
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| 存储: |
Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**
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| 存储缓冲液: |
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.05% sodium azide.
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| 注意事项: |
This product is for research use only, not for diagnostic or therapeutic use.
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