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Rabbit Anti-Human Dok-4 Polyclonal Antibody
Rabbit Anti-Human Dok-4 Polyclonal Antibody


生产种属

Human (Homo sapiens)

来源

Polyclonal antibody preparation

宿主

Rabbit

同义词

DOK4; Docking protein 4; Downstream of tyrosine kinase 4; Insulin receptor substrate 5; IRS-5; IRS5

浓度

1 mg/mL

形态

Lyophilized or Liquid

纯度

> 90% as determined by SDS-PAGE

分子质量

37 kDa

亚型

IgG

纯化方法

Rabbit Polyclonal IgG, the antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

标记

None

免疫原

The antiserum was produced against synthesized peptide derived from human Dok-4. AA range: 11-60 (Q8TEW6).

应用

WB; IHC; IF; ELISA

货号

PA85947HU

规格

100μg 500μg

价格

查询

货期

3-5个工作日

说明书

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产品概述:
DOK4 is rapidly and heavily phosphorylated in response to insulin and, once phosphorylated, binds a set of SH2 domain proteins, including RasGAP, CRK, and the nonreceptor tyrosine kinases SRS and FYN. DOK4 also activates MAPK in cells. In contrast, DOK5 is phosphorylated much more slowly and does not associate with this set of SH2 homology domains or activate MAPK. In addition to the PH and PTB domains, DOK4 contains a short C terminus with 5 potential sites for tyrosine phosphorylation. By RT-PCR analysis, Favre et al. (2003) detected expression of DOK4 and DOK5 in peripheral blood T cells. They noted that DOK5 is expressed in activated but not resting T cells, suggesting that the expression of DOK5 is regulated upon T cell activation.

特异性:
Dok-4 Polyclonal Antibody detects endogenous levels of Dok-4 protein.
使用方法:
ELISA=1:5000-20000; WB=1:500-2000; IHC=1:100-300; IF=1:200-1000; Not yet tested in other applications.
稳定性:
Store at -20°C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-8°C.
存储:
Shipped at 2-8°C, Store it under sterile conditions at -20°C to -80°C upon receiving. **Avoid repeated freeze-thaw cycles.**
存储缓冲液:
0.01M TBS (pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
注意事项:
This product is for research use only, not for diagnostic or therapeutic use.