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Chicken Interferon γ (IFN-γ) ELISA Kit

  • Cat.No.: AE38822CH

  • Reactivity: Chicken (Gallus)

To Purchase AE38822CH

Size:
48T 96T
Price: 查询

Reagent Preparation







Results demonstration







Assay Procedure Summary







Certificate



Product Details

种属 Chicken (Gallus)
UniProt号 P49708
缩写 IFN-
别名
线性范围 3.12-200 ng/mL
灵敏度 1.56 ng/mL
样本类型
检测方法 Sandwich
分析方法
反应时间
样本体积
检测波长

原理


 

产品概述


 

组份


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 10 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

 

特异性


 

回收率


Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

精密度


 

线性


Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

稳定性


 

样本收集及储存


 

注意事项


 
  1. Mortality, growth, and egg production do not differ between non-transgenic and transgenic female chickens with ubiquitous expression of the 3D8 single chain variable fragment gene IF: 3.352
    To date, many transgenic (TG) chicken lines have been developed, but few studies have performed a comparative analysis of their mortality, growth, and egg productivity.
  2. Administration of L. salivarius expressing 3D8 scFv as a feed additive improved the growth performance, immune homeostasis, and gut microbiota of chickens IF: 1.301
    Probiotics have been defined as live microorganisms that are administered in an appropriate amount to provide health benefits to the host animal.
  3. Oral administration of Lactobacillus reuteri expressing a 3D8 single-chain variable fragment (scFv) enhances chicken growth and conserves immune homeostasis IF: 1.497
    The present study was aimed to investigate the effects of 3D8 scFv-secreting Probiotic Lactobacillus reuteri (L. reuteri) on growth performance, inflammatory responses, and intestinal microbial flora in chickens.