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Chicken Interferon γ (IFN-γ) ELISA Kit

Citations(4) Uniprot : P49708
  • Cat.No.: AE38822CH

  • Reactivity: Chicken (Gallus)

To Purchase AE38822CH

Size:
48T 96T
Price: 查询

Reagent Preparation







Results demonstration







Assay Procedure Summary







Certificate



Product Details

种属 Chicken (Gallus)
UniProt号 P49708
缩写 IFN-
别名
线性范围 3.12-200 ng/mL
灵敏度 1.56 ng/mL
样本类型
检测方法 Sandwich
分析方法
反应时间
样本体积
检测波长

原理


 

产品概述


 

组份


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 12 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 12 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 10 mL

Stop Solution

1 x 6 mL

Adhesive Films

4

 

特异性


 

回收率


Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

精密度


 

线性


Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

稳定性


 

样本收集及储存


 

注意事项


 
  1. Comparative analyses of serum parameters between nontransgenic and transgenic male chickens expressing the 3D8 single-chain variable fragment gene, and mortality and growth characteristics IF: 2.500
    Transgenic (TG) animals offer significant potential for diverse applications but may pose risks if the impact of transgene expression on health and physiological parameters is not thoroughly assessed.
  2. Mortality, growth, and egg production do not differ between non-transgenic and transgenic female chickens with ubiquitous expression of the 3D8 single chain variable fragment gene IF: 3.352
    To date, many transgenic (TG) chicken lines have been developed, but few studies have performed a comparative analysis of their mortality, growth, and egg productivity.
  3. Administration of L. salivarius expressing 3D8 scFv as a feed additive improved the growth performance, immune homeostasis, and gut microbiota of chickens IF: 1.301
    Probiotics have been defined as live microorganisms that are administered in an appropriate amount to provide health benefits to the host animal.
  4. Oral administration of Lactobacillus reuteri expressing a 3D8 single-chain variable fragment (scFv) enhances chicken growth and conserves immune homeostasis IF: 1.497
    The present study was aimed to investigate the effects of 3D8 scFv-secreting Probiotic Lactobacillus reuteri (L. reuteri) on growth performance, inflammatory responses, and intestinal microbial flora in chickens.