Semicarbazidehydro chloride (AMOZ) ELISA Kit

This assay is based on the competitive enzyme immunoassay for the detection of AMOZ in the sample. The coupling antigens are pre-coated on the micro-well stripes. The AMOZ in the sample and the coupling antigens pre-coated on the micro-well stripes compete for the anti-AMOZ antibodies. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the AMOZ in it. This value is compared to the standard curve and the AMOZ concentration is subsequently obtained.
 

AMOZ was the father of the prophet Isaiah, mentioned in Isaiah 1:1 and 2:1, and in II Kings 19:2, 20; 20:1. Nothing else is known for certain about him. There is a Talmudic tradition that when the name of a prophet's father is given, the father was also a prophet, so that AMOZ would have been a prophet like his son. Though it is mentioned frequently as the patronymic title of Isaiah, the name AMOZ appears nowhere else in the Bible. The rabbis of the Talmud declared, based upon a rabbinic tradition, that Amoz was the brother of Amaziah, the king of Judah at that time (and, as a result, that Isaiah himself was a member of the royal family). According to one tradition, AMOZ is the “man of God” in 2 Chronicles 25:7-9, who cautioned Amaziah to release the Israelite mercenaries that he had hired.
 

Reagents	Quantity	Reagents	Quantity
Assay plate (96 Wells)	1	Instruction manual	1
Standard	6 x 1 mL	Redissolving Solution (concentrate 2 x)	2 x 20 mL
Antibody	1 x 7 mL	HRP-Conjugate	1 x 7 mL
Wash Buffer (concentrate 20 x)	2 x 20 mL	Stop Solution	1 x 7 mL
Substrate A	1 x 7 mL	Substrate B	1 x 7 mL
2-Nitrobenzaldehyde (C7H5NO3)	1 x 10 mL	Adhesive Films	4

 

 

Sample Type	Number	Recovery range (%)	Average(%)
Serum	10	90-101	96
EDTA plasma	10	89-97	93
Heparin plasma	10	91-99	95

 

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

Sample Type	1：2	1：4	1：8	1：16
Serum	78-89%	81-99%	92-103%	95-105%
EDTA plasma	91-101%	90-98%	93-101%	91-98%
Heparin plasma	92-103%	93-102%	92-99%	91-101%

 

The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
 

Store at 2-8°C. Please refer to Instruction Manual.
 

Store at 2-8°C. Please refer to Instruction Manual.