Product Details
种属 |
N/A |
UniProt号 |
N/A |
缩写 |
DES |
别名 |
N/A |
线性范围 |
0.025-2.025 ppb |
灵敏度 |
0.025 ppb |
样本类型 |
Urine, tissue, feed, etc. |
检测方法 |
Competitive ELISA |
分析方法 |
Quantitive |
反应时间 |
1-3h |
样本体积 |
10-200 μL |
检测波长 |
450 nm |
原理
This assay is based on the competitive enzyme immunoassay for the detection of DES in the sample. The conjugate antigen is pre-coated on the micro-well stripes. The DES in the sample competes with the conjugate antigen pre-coated on the micro-well stripes, to interact with the antibodies against DES. After the addition of the enzyme conjugate, the TMB substrate is added for coloration. The optical density (OD) value of the sample has a negative correlation with the content of DES in the sample. This value is compared to the standard curve and the DES concentration is subsequently obtained.
产品概述
Diethylstilbestrol (DES, former BAN stilboestrol) is a synthetic nonsteroidal estrogen that was first synthesized in 1938. It is also classified as an endocrine disruptor. Human exposure to DES occurred through diverse sources, such as dietary ingestion from supplemented cattle feed and medical treatment for certain conditions, including breast and prostate cancers. From about 1940 to 1971, DES was given to pregnant women in the mistaken belief it would reduce the risk of pregnancy complications and losses.
In 1971, DES was shown to cause a rare vaginal tumor in girls and women who had been exposed to this drug in utero. The United States Food and Drug Administration subsequently withdrew DES from use in pregnant women. Follow-up studies have indicated that DES also has the potential to cause a variety of significant adverse medical complications during the lifetimes of those exposed. The United States National Cancer Institute recommends women born to mothers who took DES undergo special medical exams on a regular basis to screen for complications as a result of the drug. Individuals who were exposed to DES during their mothers' pregnancies are commonly referred to as “DES daughters” and “DES sons”.
组份
Reagents | Quantity | Reagents | Quantity |
Assay plate (96 Wells) | 1 | Instruction manual | 1 |
Standard | 6 x 1 mL | Redissolving Solution (concentrate 5 x) | 2 x 20 mL |
Antibody | 1 x 7 mL | HRP-Conjugate | 1 x 12 mL |
Wash Buffer (concentrate 20 x) | 2 x 20 mL | Stop Solution | 1 x 7 mL |
Substrate A | 1 x 7 mL | Substrate B | 1 x 7 mL |
Adhesive Films
| 4 |
|
|
回收率
Sample Type |
Number |
Recovery range (%) |
Average(%) |
Serum |
10 |
90-101 |
96 |
EDTA plasma |
10 |
89-97 |
93 |
Heparin plasma |
10 |
91-99 |
95 |
精密度
Intra-assay Precision (Precision within an assay)
Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.
Inter-assay Precision (Precision between assays)
Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.
CV (%) = SD/meanX100
Intra-Assay: CV<8%
Inter-Assay: CV<12%
线性
Sample Type |
1:2 |
1:4 |
1:8 |
1:16 |
Serum |
78-89% |
81-99% |
92-103% |
95-105% |
EDTA plasma |
91-101% |
90-98% |
93-101% |
91-98% |
Heparin plasma |
92-103% |
93-102% |
92-99% |
91-101% |
稳定性
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
样本收集及储存
Store at 2-8°C. Please refer to Instruction Manual.
注意事项
Store at 2-8°C. Please refer to Instruction Manual.