Product Details
生产种属 |
Human |
来源 |
Prokaryotic expression |
宿主 |
E. coli |
同义词 |
ALP; ALKP; ALPase; Alk Phos |
浓度 |
1 mg/mL |
形态 |
Lyophilized or Liquid |
纯度 |
> 95% as determined by SDS-PAGE |
序列 |
B2R6Q2 |
标签 |
N-terminal His-tag |
纯化方式 |
Affinity purification |
分子量 |
15 kDa |
应用 |
Western blotting,ELISA;SDS-PAGE;Antigen; |
产品概述
Alkaline phosphatase (ALP, ALKP, ALPase, Alk Phos) (EC 3.1.3.1) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase.
使用方法
Reconstitute in PBS or others.
稳定性
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存储
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
存储缓冲液
Lyophilized from 50mM Tris-HCL,0.5mM EDTA,50mM NaCl,pH 7.4
注意事项
This product is for research use only, not for diagnostic or therapeutic use.