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Recombinant Human Myeloperoxidase (MPO) Protein

  • Cat.No.: AP50046HU

  • Reactivity: Human

To Purchase AP50046HU

Size:
100μg 500μg
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Product Details

生产种属 Human
来源 Prokaryotic expression
宿主 E. coli
同义词 N/A
浓度 1 mg/mL
形态 Lyophilized or Liquid
纯度 > 95% as determined by SDS-PAGE
序列 N/A
标签 N/A
纯化方式 Affinity purification
分子量 14.4 kDa
应用 Western blotting,ELISA;SDS-PAGE;Antigen;

产品概述

Myeloperoxidase (MPO) is a hemoprotein that is abundantly expressed in polymorphonuclear leukocytes (neutrophils) and secreted during their activation. The presence of a peroxidase in the cytoplasmic granules of leukocytes was suggested at the beginning of 20th century but it was the early 1940s that it was purified for the first time. Native MPO is a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 – 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW about 150 kDa and theoretical pI 9.2. MPO plays an important role in neutrophil microbicidal action through catalyzing chloride ion oxidation to hypochlorous acid, which is a potent antimicrobial agent.
 

使用方法

Reconstitute in PBS or others.
 

稳定性

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
 

存储

Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
 

存储缓冲液

The protein solution is in 0.05M phosphate buffer containing 0.15M NaCl and 0.09% NaN3 pH 7.5. Filtered through a 0.8μM membrane
 

注意事项

This product is for research use only, not for diagnostic or therapeutic use.