Product Details
生产种属 |
Human |
来源 |
Prokaryotic expression |
宿主 |
E. coli |
同义词 |
bHLHe78; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8 |
浓度 |
1 mg/mL |
形态 |
Lyophilized or Liquid |
纯度 |
> 95% as determined by SDS-PAGE |
序列 |
Gln 717-Asn 826 (NP_001521.1) |
标签 |
N-terminal His-tag |
纯化方式 |
Affinity purification |
分子量 |
13.1 kDa |
应用 |
Western blotting,ELISA;SDS-PAGE;Antigen; |
产品概述
Hypoxia-inducible factor 1-alpha, also known as HIF-1-alpha, is a protein that in humans is encoded by the HIF1A gene.Two alternative transcripts encoding different isoforms have been identified.The protein encoded by HIF1 is a bHLH - PAS transcription factor found in mammalian cells growing at low oxygen concentrations. It plays an essential role in cellular and systemic responses to hypoxia. This is one of the class of hypoxia inducible factors, a family that includes Hif1a, Hif2a, and Hif3a.
使用方法
Reconstitute in PBS or others.
稳定性
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存储
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
存储缓冲液
Lyophilized from 20mM PB, 300mMNaCl,pH7.7
注意事项
This product is for research use only, not for diagnostic or therapeutic use.