Product Details
生产种属 |
Human |
来源 |
Prokaryotic expression |
宿主 |
E. coli |
同义词 |
PADI2; PAD-H19; PAD2; PDI2 |
浓度 |
1 mg/mL |
形态 |
Lyophilized or Liquid |
纯度 |
> 90% as determined by SDS-PAGE |
序列 |
Leu1~Ala109 (Q9Y2J8) |
标签 |
N-terminal His-tag |
纯化方式 |
Affinity purification |
分子量 |
13 kDa |
应用 |
Western blotting,ELISA;SDS-PAGE;Antigen; |
产品概述
Protein-arginine deiminase type-2 is an enzyme that in humans is encoded by the PADI2 gene.
This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. The type II enzyme is the most widely expressed family member. Known substrates for this enzyme include myelin basic protein in the central nervous system and vimentin in skeletal muscle and macrophages. This enzyme is thought to play a role in the onset and progression of neurodegenerative human disorders, including Alzheimer disease and multiple sclerosis, and it has also been implicated in glaucoma pathogenesis. This gene exists in a cluster with four other paralogous genes.
使用方法
Reconstitute in PBS or others.
稳定性
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存储
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
存储缓冲液
Lyophilized from 50mM Tris-HCL,0.5mM EDTA,300mM NaCl,pH 9.0
注意事项
This product is for research use only, not for diagnostic or therapeutic use.