Product Details
生产种属 |
Human |
来源 |
Prokaryotic expression |
宿主 |
E. coli |
同义词 |
UBE2C; UBCH10; dJ447F3.2 |
浓度 |
1 mg/mL |
形态 |
Lyophilized or Liquid |
纯度 |
> 95% as determined by SDS-PAGE |
序列 |
Ala70-Pro180 (O00762) |
标签 |
N-terminal His-tag |
纯化方式 |
Affinity purification |
分子量 |
13 kDa |
应用 |
Western blotting,ELISA;SDS-PAGE;Antigen; |
产品概述
Ubiquitin-conjugating enzyme E2 C is a protein that in humans is encoded by the UBE2C gene.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for the destruction of mitotic cyclins and for cell cycle progression. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.
使用方法
Reconstitute in PBS or others.
稳定性
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
存储
Store it under sterile conditions at -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. Samples are stable for up to twelve months from date of receipt at -80℃.
存储缓冲液
Lyophilized from 50mM Tris-HCL,0.5mM EDTA,300mM NaCl,pH 9.0
注意事项
This product is for research use only, not for diagnostic or therapeutic use.