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Cat Deoxypyridinoline (DPD) ELISA Kit

Citations(1)Uniprot : N/A
  • Cat.No.: AE64670CA

  • Reactivity: Cat (Felis catus,Feline)

To Purchase AE64670CA

Size:
48T 96T
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Reagent Preparation







Results demonstration







Assay Procedure Summary







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Product Details

种属 Cat (Felis catus,Feline)
UniProt号 N/A
缩写 DPD
别名 N/A
线性范围 1.56-100 ng/mL
灵敏度 1 ng/mL
样本类型 Serum, Plasma, Other biological fluids
检测方法 Sandwich
分析方法 Quantitive
反应时间 1-4.5h
样本体积 1-200 μL
检测波长 450 nm

原理

This assay employs a two-site sandwich ELISA to quantitate DPD in samples. An antibody specific for DPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPD bound in the initial step. The color development is stopped and the intensity of the color is measured.
 

产品概述

Deoxypyridinoline (Dpd) is one of the two pyridinium cross-links that provide structural rigidity to type I collagen in bone. During osteoclastic resorption, Dpd is released into circulation and is excreted in the urine in free and peptide-bound forms.Deoxypyridinoline (Dpd) occurs mainly in Type I collagen of bone. After the formation of the Type I collagen matrix, the action of lysyl oxidase on two hydroxylysine and a lysine residue result in the formation of a Dpd cross link between two triple helical strands of collagen. Deoxypyridinoline (Dpd) has been described as a marker of bone turnover in metastatic breast cancer.Pyridinoline (PYD) and Deoxypyridinoline (DPD) are the major stable cross-links. These cross-links, primarily in bone and cartilage, have been found in all the connective tissues except the skin. PYD is the major component of these tissues, whereas DPD has only been detected in bone and dentine.
 

组份


Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1 x 20 mL

Biotin-Conjugate (concentrate 100 x)

1 x 120 μL

Biotin-Conjugate Diluent

1 x 20 mL

Streptavidin-HRP  (concentrate 100 x)

1 x 120 μL

Streptavidin-HRP Diluent   

1 x 20 mL

Wash Buffer (concentrate 25 x)

1 x 20 mL

Substrate Solution

1 x 10 mL

Stop Solution

1 x 10 mL

Adhesive Films

4

 

特异性

This assay has high sensitivity and excellent specificity for detection of Human DPD. No significant cross-reactivity or interference between Human DPD and analogues was observed.
 

回收率

Matrices listed below were spiked with certain level of recombinant Human DPD and the recovery rates were calculated by comparing the measured value to the expected amount of Human DPD in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin plasma

10

91-99

95

 

精密度

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

线性

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin plasma

92-103%

93-102%

92-99%

91-101%

 

稳定性

Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
 

样本收集及储存

Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. 
Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles.
Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.

 

注意事项

Store at 2-8°C. Please refer to Instruction Manual.
 
  1. Effects of Erythropoietin-Promoted Fracture Healing on Bone Turnover Markers in Cats IF: 4.800
    In orthopaedics, erythropoietin (EPO) is applied in the preoperative management of anaemic patients, but also as a stimulating factor to assist bone regeneration due to its angiogenic and osteoinductive potential.